versadoc 4000 gel Search Results


amolecular imaging apparatus  (Bio-Rad)
96
Bio-Rad amolecular imaging apparatus
Amolecular Imaging Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
amolecular imaging apparatus - by Bioz Stars, 2026-09
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gel image  (Bio-Rad)
96
Bio-Rad gel image
Gel Image, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Computer/10__1007_slash_s11756___024___01753___y-44-12-27
Average 96 stars, based on 1 article reviews
gel image - by Bioz Stars, 2026-09
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flamingo fluorescent gel stain  (Bio-Rad)
94
Bio-Rad flamingo fluorescent gel stain
Flamingo Fluorescent Gel Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Flamingo+Fluorescent+Protein+Gel+Stain/pmc03979864-361-4-8
Average 94 stars, based on 1 article reviews
flamingo fluorescent gel stain - by Bioz Stars, 2026-09
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ethidium bromide  (Bio-Rad)
96
Bio-Rad ethidium bromide
Ethidium Bromide, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Ethidium+Bromide+Solution/pmc02938363-123-5-22
Average 96 stars, based on 1 article reviews
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sypro ruby protein gel stain  (Bio-Rad)
94
Bio-Rad sypro ruby protein gel stain
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Sypro Ruby Protein Gel Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/SYPRO+Ruby+Protein+Gel+Stain/pmc03405509-236-10-15
Average 94 stars, based on 1 article reviews
sypro ruby protein gel stain - by Bioz Stars, 2026-09
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sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page  (Bio-Rad)
97
Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/SDS+(Sodium+Dodecyl+Sulfate)/pm21475896-32-8-30
Average 97 stars, based on 1 article reviews
sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page - by Bioz Stars, 2026-09
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imagelab software  (Bio-Rad)
99
Bio-Rad imagelab software
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Imagelab Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Image+Lab+Software/pmc05737570-90-25-27
Average 99 stars, based on 1 article reviews
imagelab software - by Bioz Stars, 2026-09
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tween 20  (Bio-Rad)
98
Bio-Rad tween 20
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Tween 20, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Tween+20/pmc07408772-245-11-21
Average 98 stars, based on 1 article reviews
tween 20 - by Bioz Stars, 2026-09
98/100 stars
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coomassie brilliant blue  (Bio-Rad)
96
Bio-Rad coomassie brilliant blue
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Coomassie Brilliant Blue, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/Coomassie+Brilliant+Blue+G-250/pm21475896-32-27-30
Average 96 stars, based on 1 article reviews
coomassie brilliant blue - by Bioz Stars, 2026-09
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pdquest 2de analysis software  (Bio-Rad)
96
Bio-Rad pdquest 2de analysis software
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Pdquest 2de Analysis Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/PDQuest+Advanced+2-D+Analysis+Software/pm41398938-108-15-19
Average 96 stars, based on 1 article reviews
pdquest 2de analysis software - by Bioz Stars, 2026-09
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anti-human cd3-ζ monoclonal antibody  (Becton Dickinson)
90
Becton Dickinson anti-human cd3-ζ monoclonal antibody
Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by <t>SYPRO</t> Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has <t>a</t> <t>molecular</t> mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.
Anti Human Cd3 ζ Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/versadoc+4000+gel/anti+human+cd3+%CE%B6+monoclonal+antibody/us09629877-822-16-25
Average 90 stars, based on 1 article reviews
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Image Search Results


Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by SYPRO Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has a molecular mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.

Journal: The Journal of Experimental Medicine

Article Title: Bicarbonate and functional CFTR channel are required for proper mucin secretion and link cystic fibrosis with its mucus phenotype

doi: 10.1084/jem.20120562

Figure Lengend Snippet: Stimulation of mucus secretion. (A) Mucus thickness of ileal explant mounted in the horizontal chamber. After mounting (time 0), mucus was removed from the CF mice by extensive aspiration before the measurement at 20 min. Mucus secretion was stimulated by basal perfusion with PGE 2 and CCh for 40 min (WT, CF, n = 4; WT CCh-PGE 2 , n = 8; CF CCh-PGE 2 , n = 7; **, P = 0.008). (B) Total mucus thickness after 40-min stimulation with CCh and PGE 2 and remaining thickness after aspiration of the mucus (n.s., nonsignificant; WT, n = 7; CF, n = 6; ***, P = 0.0006). (C) Quantification of Muc2 amount in secreted mucus from WT and CF ileum. The secreted mucus was collected, analyzed on an SDS-agarose-polyacrylamide composite gel, and visualized by SYPRO Ruby (top) and Alcian blue (bottom). A representative gel of four repeated experiments is shown. The Muc2 monomeric band is indicated by the arrows and has a molecular mass of ∼2.5 MD. (D) Intensity of the SYPRO Ruby staining of the Muc2 monomeric band in CF ( n = 6) and WT ( n = 6; four gels; **, P = 0.002). Data are presented as mean ± SEM.

Article Snippet: For quantification of the mucins, the gel was stained with SYPRO Ruby protein gel stain (Bio-Rad Laboratories), bands were quantified using a Molecular Imager VersaDoc MP 4000 reader (Bio-Rad Laboratories), and intensities were analyzed using Image Laboratory Software version 3.0 (Bio-Rad Laboratories).

Techniques: Staining